Immunoassay Reagents and Kits

Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.

We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.

ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.

Life Science Research ELISA Kits (132,532)

Singleplex and Multiplex Bead Based Immunoassays (5,858)

ELISA Substrates and Reagents (2,325)

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586 – 600 of 138,172 results

586

Invitrogen™ Human IL-8 Rapid ELISA Kit

ELISA

Pricing & Availability
Specifications

Shipping Condition	Wet or Dry Ice
Immunoassay Kit Format	Coated ELISA Kit
Target Species	Human
Conjugate	HRP
Interassay CV	<10%
Intraassay CV	<10%
Gene Symbol	CXCL8
Total Assay Time	1 hr 30 min
Assay Sensitivity	9.38 pg/mL
Storage Requirements	Refer to product documentation for component specific storage temperature.
Assay Range	15.63-1000 pg/mL
Test Time	15 min
Accession Number	P10145
Regulatory Status	RUO
Target	IL-8
Kit Contents	Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer,Standard,HRP Conjugate,Standard & Sample Diluent,HRP Conjugate Diluent,Wash Buffer,TMB Substrate,Stop Solution,Plate Sealer
Gene Alias	(Ala-IL-8)77; (Ser-IL-8)72; Alveolar macrophage chemotactic factor I; alveolar macrophage-derived chemotactic factor-I; AMCF-I; ANX7; beta endothelial cell-derived neutrophil activating peptide; beta-thromboglobulin-like protein; chemokine (C-X-C motif) ligand 8; C-X-C motif chemokine 8; C-X-C motif chemokine ligand 8; CXCL8; cytokine 8; emoctakin; GCP/IL-8 protein I; GCP/IL-8 protein II; GCP/IL-8 protein III; GCP/IL-8 protein IV; GCP/IL-8 protein V; GCP/IL-8 protein VI; GCP1; GCP-1; Granulocyte chemotactic protein 1; IL8; IL-8; IL-8(1-77); IL-8(5-77); IL-8(6-77); IL-8(7-77); IL-8(8-77); IL-8(9-77); IL8/NAP1 form I; IL8/NAP1 form II; IL8/NAP1 form III; IL8/NAP1 form IV; IL8/NAP1 form V; IL8/NAP1 form VI; ILN; Interleukin; interleukin 8; Interleukin8; interleukin-8; interleuklin-8 precursor; LECT; LUCT; lung giant cell carcinoma-derived chemotactic protein; lymphocyte derived neutrophil activating peptide; Lymphocyte-derived neutrophil-activating factor; LYNAP; MDNCF; MDNCF-a; MDNCF-b; MDNCF-c; MONAP; Monocyte-derived neutrophil chemotactic factor; monocyte-derived neutrophil-activating peptide; NAF; NAP1; NAP-1; neutrophil activating peptide 1; neutrophil and T-lymphocyte chemotactic and activating factor; Neutrophil attractant/activation protein 1; neutrophil attractant/activation protein-1; Neutrophil-activating factor; neutrophil-activating peptide 1; Neutrophil-activating protein 1; Permeability factor 1; PF1; Protein 3-10C; RP11-537A6.8; RPF1; small inducible cytokine subfamily B, member 8; SNX; SYNEXIN; T-cell chemotactic factor; tumor necrosis factor-induced gene 1(Ala-IL-8)77; (Ser-IL-8)72; Alveolar macrophage chemotactic factor I; alveolar macrophage-derived chemotactic factor-I; AMCF-I; ANX7; beta endothelial cell-derived neutrophil activating peptide; beta-thromboglobulin-like protein; chemokine (C-X-C motif) ligand 8; C-X-C motif chemokine 8; C-X-C motif chemokine ligand 8; CXCL8; cytokine 8; emoctakin; GCP/IL-8 protein I; GCP/IL-8 protein II; GCP/IL-8 protein III; GCP/IL-8 protein IV; GCP/IL-8 protein V; GCP/IL-8 protein VI; GCP1; GCP-1; Granulocyte chemotactic protein 1; IL8; IL-8; IL-8(1-77); IL-8(5-77); IL-8(6-77); IL-8(7-77); IL-8(8-77); IL-8(9-77); IL8/NAP1 form I; IL8/NAP1 form II; IL8/NAP1 form III; IL8/NAP1 form IV; IL8/NAP1 form V; IL8/NAP1 form VI; ILN; Interleukin; interleukin 8; Interleukin8; interleukin-8; interleuklin-8 precursor; LECT; LUCT; lung giant cell carcinoma-derived chemotactic protein; lymphocyte derived neutrophil activating peptide; Lymphocyte-derived neutrophil-activating factor; LYNAP; MDNCF; MDNCF-a; MDNCF-b; MDNCF-c; MONAP; Monocyte-derived neutrophil chemotactic factor; monocyte-derived neutrophil-activating peptide; NAF; NAP1; NAP-1; neutrophil activating peptide 1; neutrophil and T-lymphocyte chemotactic and activating factor; Neutrophil attractant/activation protein 1; neutrophil attractant/activation protein-1; Neutrophil-activating factor; neutrophil-activating peptide 1; Neutrophil-activating protein 1; Permeability factor 1; PF1; Protein 3-10C; RP11-537A6.8; RPF1; small inducible cytokine subfamily B, member 8; SNX; SYNEXIN; T-cell chemotactic factor; tumor necrosis factor-induced gene 1;
Product Type	ELISA
For Use With (Equipment)	Absorbance Microplate Reader
Gene ID (Entrez)	3576
Sample Type	Plasma,Serum
Label or Dye	HRP

587

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Invitrogen™ Feline IL-1 beta ELISA Kit

ELISA

Pricing & Availability
Specifications

Conjugate	HRP
Interassay CV	<12%
Intraassay CV	<10
Gene Symbol	IL1B
Total Assay Time	4 hrs 45 mins
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	16 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	16 to 4,000 pg/mL
Test Time	1 hrs 20 mins
Accession Number	catabolin; cytokine; il 1 b; IL 1ß; IL-1; IL1 B; IL-1 beta; IL-1 precursor; IL1B; Il-1 b; IL1B1; IL1beta; IL-1 beta; IL1-BETA; IL1F2; IL1ß; ILN; Interleukin; interleukin 1 beta; interleukin 1, beta; interleukin 1, beta 1; interleukin 1-beta; Interleukin1 beta; interleukin-1 beta; interleukin-1 beta precursor; interleukin-1 beta precursor (AA -113 to 153); interleukin-1 beta proprotein; interleukin-1 beta-like; Interleukin-1 b; interleukin-beta; LOC100052414; lymphocyte proliferation-potentiating factor; preinterleukin 1 beta; prointerleukin-1 beta; pro-interleukin-1-beta
Regulatory Status	RUO
Target	IL-1 beta
Kit Contents	Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	768274
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP

588

R&D Systems™ Human Vitamin D BP Quantikine ELISA Kit

Human Vitamin D BP Quantikine ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.

Pricing & Availability

589

Novus Biologicals™ Human HABP1/C1QBP/GC1q R - Ready-To-Use ELISA Kit (Colorimetric)

Colorimetric ELISA Kit

Pricing & Availability

590

Novus Biologicals Mouse CD48/SLAMF2 ELISA Kit (Colorimetric)

Pricing & Availability

591

Novus Biologicals™ u-Plasminogen Activator/Urokinase Antibody Pair [HRP]

Mouse Monoclonal Antibody Pair

Pricing & Availability
Specifications

Host Species	Mouse
Target Species	Human
Conjugate	HRP
Gene Symbol	PLAU
Assay Sensitivity	4.69-300 pg/mL
Storage Requirements	Storage is content dependent.
Research Discipline	Cancer
Assay Range	4.69 pg/mL
For Use With (Application)	Sandwich ELISA
Assay	Sandwich ELISA
Regulatory Status	RUO
Target	u-Plasminogen Activator/Urokinase
Specificity	This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human u-Plasminogen Activator/Urokinase.
Kit Contents	Standard: Each vial contains 15 ng of recombinant Human u-Plasminogen Activator/Urokinase. Capture Antibody: 1 mg/mL of mouse anti-Human u-Plasminogen Activator/Urokinase monoclonal antibody (in PBS, pH 7.4). Detection Antibody: 0.2 mg/mL of mouse anti-Human u-Plasminogen Activator/Urokinase monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
Gene Alias	ATF, EC 3.4.21, EC 3.4.21.73, plasminogen activator, urokinase, uPA, u-PA, U-plasminogen activator, urinary, urokinase-type plasminogen activator
Product Type	Antibody Pairs
Gene ID (Entrez)	5328
Technique	1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 300 pg/mL is recommended.

592

Novus Biologicals™ Human FUR1 ELISA Kit (Colorimetric)

Colorimetric ELISA Kit

Pricing & Availability

593

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Invitrogen™ Human FGF-3 ELISA Kit

ELISA

Pricing & Availability
Specifications

Conjugate	HRP
Interassay CV	<12%
Intraassay CV	<10
Gene Symbol	FGF3
Total Assay Time	4 hrs 45 mins
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	0.45 ng/mL
Storage Requirements	2°C to 8°C
Assay Range	0.45 to 100 ng/mL
Test Time	1 hrs 20 mins
Accession Number	FGF; Fgf3; FGF-3; fgf3.L; fgf3-A; Fibroblast growth factor; fibroblast growth factor 3; fibroblast growth factor 3 (murine mammary tumor virus integration site (v-int-2) oncogene homolog); fibroblast growth factor 3 L homeolog; Fibroblast growth factor-3; HBGF-3; Heparin-binding growth factor 3; Int2; INT-2; INT-2 proto-oncogene protein; interacting gene 2; Int-P; murine mammary tumor virus integration site 2, mouse; oncogene INT2; Proto-oncogene Int-2; V-INT2 murine mammary tumor virus integration site oncogene homolog; XELAEV
Regulatory Status	RUO
Target	FGF3
Kit Contents	Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	2248
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP

594

Invitrogen™ Mouse GM-CSF ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Mouse
Conjugate	HRP
Interassay CV	10%
Intraassay CV	5%
Gene Symbol	CSF2
Total Assay Time	3 hr 30 min
Sample Volume	Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	2.0 pg/mL
Storage Requirements	2-8°C
Assay Range	3.9-250 pg/mL
For Use With (Application)	ELISA
Test Time	1 hr 20 min
Accession Number	P01587
Regulatory Status	RUO
Target	GM-CSF
Kit Contents	Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	12981
Sample Type	Serum,Supernatant
Label or Dye	Biotin
Synonym	Molgramostin, Sargramostim

595

Invitrogen™ Human TSLP Uncoated ELISA Kit

ELISA

Pricing & Availability
Specifications

Immunoassay Kit Format	Uncoated ELISA Kit
Target Species	Human
Conjugate	HRP
Gene Symbol	TSLP
Total Assay Time	24 hr 30 min
Assay Sensitivity	8 pg/mL
Storage Requirements	2-8°C
Assay Range	8-1000 pg/mL
Test Time	1 hr 45 min
Accession Number	Q969D9
Regulatory Status	RUO
Target	TSLP
Kit Contents	Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, biotin-conjugated antibody, Standard: Recombinant cytokine for generating standard curve and calibrating samples, ELISA Coating Buffer Powder: This uncoated ELISA kit with plates may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 μM) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20)., Assay Diluent: 5X concentrated, Detection enzyme: Pre-titrated Avidin-HRP, Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86),Purified antibody,Detection Antibody: Pre-titrated,Biotin-conjugated antibody,Standard: Recombinant cytokine for generating standard curve and calibrating samples,ELISA Coating Buffer Powder: This uncoated ELISA kit with plates may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 μM) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).,Assay Diluent: 5X concentrated,Detection enzyme: Pre-titrated Avidin-HRP,Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
Gene Alias	Thymic stroma-derived lymphopoietin; thymic stromal lymphopoietin; TSLPThymic stroma-derived lymphopoietin; thymic stromal lymphopoietin; TSLP; IL-6
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	85480
Sample Type	Plasma,Serum,Supernatant
Label or Dye	Biotin

596

Novus Biologicals™ Sheep Luteinizing Hormone ELISA Kit (Colorimetric)

Assay has high sensitivity and specificity for detection of sheep LH

Pricing & Availability
Specifications

Target	Luteinizing Hormone
Specificity	For detection of sheep LH
Kit Contents	Assay plate, Adhesive Strip (For 96 wells), HRP-conjugate, Substrate B, Wash Buffer (20 x concentrate), Standard, Substrate A, Stop Solution, Instruction manual
Product Type	ELISA Kit (Colorimetric)
Sample Type	Plasma,Serum,Tissue Homogenates
Sample Volume	50 to 100 μL
Assay Sensitivity	0.5 mIU/ml
Assay Range	2 mIU/ml to 75 mIU/ml
Storage Requirements	Store the unopened product at 2° to 8°C. Protect from light. Do not use past expiration date.
Research Discipline	Signal Transduction
For Use With (Application)	ELISA

597

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R&D Systems™ Mouse IL-1 RI DuoSet ELISA

For the development of sandwich ELISAs to measure natural and recombinant mouse Interleukin 1 Receptor I (IL-1 RI)

Pricing & Availability

598

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Invitrogen™ Canine MCP-1 ELISA Kit

ELISA

Pricing & Availability
Specifications

Conjugate	HRP
Interassay CV	<12%
Intraassay CV	<10
Gene Symbol	Ccl2
Total Assay Time	4 hrs 45 mins
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	9 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	9 to 700 pg/mL
Test Time	1 hrs 20 mins
Accession Number	Acidic seminal fluid protein; AI323594; C-C motif chemokine 2; C-C motif chemokine ligand 2; Ccl2; chemokine (C-C motif) ligand 2; GDCF-2; HC11; H-MCP-1; HSMCR30; immediate-early serum-responsive JE protein; immediate-early serum-responsive protein JE; Je; MCAF; MCP1; MCP-1; MCP1A; MCP-1 A; MGC9434; Monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; Monocyte chemotactic protein 1; monocyte chemotactic protein 1 A; Monocyte secretory protein JE; monocyte-chemoattractant protein-1 precursor; platelet-derived growth factor-inducible protein JE; RP23-350G1.3; Scya2; Sigje; small inducible cytokine A2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); small inducible cytokine subfamily A (Cys-Cys), member 2; small inducible gene JE; small-inducible cytokine A2; SMC-CF
Regulatory Status	RUO
Target	CCL2 (MCP-1)
Kit Contents	Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	403981
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP

599

R&D Systems™ Human IFN-alpha Multi-Subtype ELISA Kit

Human IFN-alpha Multi-Subtype ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.

Pricing & Availability
Specifications

Specificity	Human IFN-alpha
Target Species	Human
Conjugate	Biotin
Product Type	Solid Phase Sandwich ELISA
Gene ID (Entrez)	3440
Sample Type	Cell Culture Supernatant
Sample Volume	Cell Culture Supernates (100 μL)
Synonym	IFNA, IFNA2, IFNA2b, IFNalpha, IFN-alphaA, INFA2, interferon alpha 2, LeIF D
Assay Sensitivity	12.5pg/mL
Storage Requirements	Store the unopened product at 2° to 8°C. Do not use past expiration date.
Assay Range	12.50 to 500 pg/mL (Cell Culture Supernates), 156.00 to 5000 pg/mL (Cell Culture Supernates)
For Use With (Application)	ELISA
Cross Reactivity	<50% cross-species reactivity observed with species tested. <0.5% cross-reactivity observed with available related molecules.

600

Invitrogen™ Human IL-10 Instant ELISA™ Kit

ELISA

Pricing & Availability

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Novus Biologicals™ Human HABP1/C1QBP/GC1q R - Ready-To-Use ELISA Kit (Colorimetric) Small and Specialty Supplier PartnerSmall and/or specialty supplier based on Federal laws and SBA requirements.Learn More

Novus Biologicals Mouse CD48/SLAMF2 ELISA Kit (Colorimetric) Small and Specialty Supplier PartnerSmall and/or specialty supplier based on Federal laws and SBA requirements.Learn More

Novus Biologicals™ u-Plasminogen Activator/Urokinase Antibody Pair [HRP] Small and Specialty Supplier PartnerSmall and/or specialty supplier based on Federal laws and SBA requirements.Learn More

Novus Biologicals™ Human FUR1 ELISA Kit (Colorimetric) Small and Specialty Supplier PartnerSmall and/or specialty supplier based on Federal laws and SBA requirements.Learn More

Novus Biologicals™ Sheep Luteinizing Hormone ELISA Kit (Colorimetric) Small and Specialty Supplier PartnerSmall and/or specialty supplier based on Federal laws and SBA requirements.Learn More

Novus Biologicals™ Human HABP1/C1QBP/GC1q R - Ready-To-Use ELISA Kit (Colorimetric)

Novus Biologicals Mouse CD48/SLAMF2 ELISA Kit (Colorimetric)

Novus Biologicals™ u-Plasminogen Activator/Urokinase Antibody Pair [HRP]

Novus Biologicals™ Human FUR1 ELISA Kit (Colorimetric)

Novus Biologicals™ Sheep Luteinizing Hormone ELISA Kit (Colorimetric)